Part:BBa_K2845042:Design
OmpR fused the N-terminus of split EPIC luciferase
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
OmpR is fused to the N-terminus of split EPIC Luciferase. OmpR is part of the natural response system from E. coli reacting to osmotic changes and inducing adaptive changes in the membrane. Only when phosphorylated by EnvZ, it has an increased binding affinity to its binding site and activates the expression of membrane proteins. This biobrick is designed to be used in a system together with a fusion protein consisting of the C-terminus of the split EPIC luciferase fused to TetR and the binding sites of OmpR and TetR placed next to each other on a high copy plasmid. Since TetR is constitutively binding to its binding site, the two luciferase halves are only brought into proximity when OmpR gets phosphorylated and binds to its binding site. This allows the visualization of transcription factor binding by circumventing the time-consuming step of transcription and translation.